Antibody for porcine reproductive and respiratory syndrome virus and uses thereof
AssigneeNovascope Biochips Inc.
InventorsChang-Fu Kuo, Ming-Tang Chiou, Wei-Hao Lin, Lian-Chin Wang, Ao-Ho Hsieh
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Abstract
The instant disclosure discloses an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and uses of such antibody or antigen-binding fragment thereof to create immunoassay methods or devices for PRRSV detection.
Claims20
An antibody or antigen-binding fragment thereof for binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the antibody or antigen-binding fragment thereof comprising:
- a heavy chain variable domain comprising:
- a heavy chain complementarity-determining region 1 (CDR-H1) comprising the sequence of SEQ ID NO:
- 1,
- a CDR-H2 comprising the sequence of SEQ ID NO:
- 2, and
- a CDR-H3 comprising the sequence of SEQ ID NO:
- 3;
- and
- a light chain variable domain comprising:
- a light chain complementarity-determining region 1 (CDR-L1) comprising the sequence of SEQ ID NO:
- 4,
- a CDR-L2 comprising the sequence of Ala-Ala-Ser, and
- a CDR-L3 comprising the sequence of SEQ ID NO:
- 5.
A biological field-effect transistor (Bio-FET), comprising:
- a transistor region;
- and
- a detecting region, wherein the detecting region comprises a detecting surface that is functionalized with an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), wherein the antibody or antigen-binding fragment thereof comprises:
- a heavy chain variable domain comprising a heavy chain complementarity-determining region 1 (CDR-H1) comprising the sequence of SEQ ID NO:
- 1, a CDR-H2 comprising the sequence of SEQ ID NO:
- 2, and a CDR-H3 comprising the sequence of SEQ ID NO:
- 3;
- and
- a light chain variable domain comprising a light chain complementarity-determining region 1 (CDR-L1) comprising the sequence of SEQ ID NO:
- 4, a CDR-L2 comprising the sequence of Ala-Ala-Ser, and a CDR-L3 comprising the sequence of SEQ ID NO:
- 5.
A method for detecting Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by using a biological field-effect transistor (Bio-FET), the method comprising:
- (a) contacting a sample with an antibody or antigen-binding fragment thereof immobilized on a detecting surface of the Bio-FET, wherein the antibody or antigen-binding fragment thereof comprises:
- a heavy chain variable domain comprising a heavy chain complementarity-determining region 1 (CDR-H1) comprising the sequence of SEQ ID NO:
- 1, a CDR-H2 comprising the sequence of SEQ ID NO:
- 2, and a CDR-H3 comprising the sequence of SEQ ID NO:
- 3;
- and
- light chain variable domain comprising a CDR-L1 comprising the sequence of SEQ ID NO:
- 4, a CDR-L2 comprising the sequence of Ala-Ala-Ser, and a CDR-L3 comprising the sequence of SEQ ID NO:
- 5,
- wherein the antibody or antigen-binding fragment thereof binds to PRRSV;
- and
- (b) analyzing an electric signal obtained from the Bio-FET.
Dependencies are inferred from the claim text.