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US 12,625,130 B2Granted
Nanopore sensor devices
AssigneeIllumina, Inc.
InventorsBoyan Boyanov, Jeffrey G. Mandell, Seth M. McDonald
Life of this patent
Estimating the term…
Abstract
An example of a nanopore sensor device includes one or more cis wells; a cis electrode; a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a lipid/solid-state membrane having a nanopore; a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells; a first concentration of an electrolyte within the one or more cis wells; and a second concentration of the electrolyte within the trans wells, wherein the first concentration is higher than the second concentration.
Claims23
A nanopore sensor device, comprising:
- one or more cis wells;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a membrane selected from the group consisting of a lipid bilayer, a solid-state membrane, and a liquid film material, and having a nanopore;
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells;
- a first concentration of an electrolyte within the one or more cis wells;
- a second concentration of the electrolyte within the trans wells, wherein the first concentration is higher than the second concentration;
- a stimulus source coupled to each of the plurality of trans electrodes either individually or via multiplexing, wherein the stimulus source is to cause current to flow through the nanopore;
- and
- a controller coupled to the stimulus source, the controller configured to individually/selectively address the plurality of trans electrodes to cause an ionic current through the nanopore of an addressed trans electrode of the plurality of trans wells;
- wherein the ionic current comprises an amount of anions of the electrolyte translocating through the nanopore to the addressed trans well that is higher than an amount of cations of the electrolyte translocating through the nanopore from the addressed trans well.
7 more elements
A nanopore sensor device, comprising:
- one or more cis wells;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a membrane selected from the group consisting of a lipid bilayer, a solid-state membrane, and a liquid film material, and having a nanopore;
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells;
- a first concentration of an electrolyte within the one or more cis wells;
- and
- a second concentration of the electrolyte within the trans wells, wherein the first concentration is higher than the second concentration;
- wherein a ratio of the first concentration to the second concentration ranges from about 10:1 to about 3:1.
5 more elements
A nanopore sensor kit, comprising:
- a nanopore sensor device, including:
- one or more cis wells including a fluid inlet;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a membrane selected from the group consisting of a lipid bilayer, a solid-state membrane, and a liquid film material, and having a nanopore;
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells;
- and
- a first concentration of an electrolyte within the one or more cis wells and the plurality of trans well;
- a second concentration of the electrolyte to be introduced into the one or more cis wells through the fluid inlet such that the one or more cis wells contain the second concentration of the electrolyte and the plurality of trans wells contain the first concentration of the electrolyte at an initial cycle of the nanopore sensor device, wherein the second concentration is higher than the first concentration;
- a stimulus source coupled to each of the plurality of trans electrodes either individually or via multiplexing, wherein the stimulus source is to cause current to flow through the nanopore;
- and
- a controller coupled to the stimulus source, the controller configured to individually/selectively address the plurality of trans electrodes to cause an ionic current through the nanopore of an addressed trans electrode of the plurality of trans wells;
- wherein the ionic current comprises an amount of anions of the electrolyte translocating through the nanopore to the addressed trans well that is higher than an amount of cations of the electrolyte translocating through the nanopore from the addressed trans well.
9 more elements
A method of detecting an ionic current to analyze a biological compound, comprising:
- providing a solid-state nanopore within a membrane separating a cis well and a trans well, the solid-state nanopore having a plurality of positively charged residues on an inner surface, and the plurality of positively charged residues being selected from the group consisting of an organic positively charged species, an inorganic positively charged species, or both organic positively charged species and inorganic positively charged species;
- providing an electrolyte within the cis well and the trans well;
- and
- applying an electric current between a cis cathode at least partially exposed to the cis well and a trans anode at least partially exposed to the trans well to generate an ionic current through the nanopore,
- wherein the plurality of positively charged residues of the nanopore inhibits translocation of cations from the trans well to the cis well during application of the electric current.
2 more elements
A method of detecting an ionic current to analyze a biological compound, comprising:
- providing a nanopore within a membrane separating a cis well and a trans well, the nanopore having a plurality of positively charged residues on an inner surface;
- providing an electrolyte within the cis well and the trans well;
- and
- applying an electric current between a cis cathode at least partially exposed to the cis well and a trans anode at least partially exposed to the trans well to generate an ionic current through the nanopore,
- wherein the plurality of positively charged residues of the nanopore inhibits translocation of cations from the trans well to the cis well during application of the electric current;
- and wherein one of:
- the electrolyte is a redox couple having a negative charge and is incorporated into a redox-inactive buffer that includes an anion having a diameter greater than a diameter of a constriction zone of the nanopore;
- and
- the electrolyte is a redox couple having a positive charge and is incorporated into a redox-inactive buffer that includes a cation having a diameter greater than a diameter of a constriction zone of the nanopore.
6 more elements
A nanopore sensor device, comprising:
- one or more cis wells;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a membrane selected from the group consisting of a lipid bilayer, a solid-state membrane, and a liquid film material, and having a nanopore;
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells;
- an electrolyte solution including:
- a redox-inactive buffer that includes a redox inactive species having a diameter greater than a diameter of a constriction zone of the nanopore;
- and
- a redox couple.
5 more elements
A nanopore sensor device, comprising:
- one or more cis wells;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a membrane selected from the group consisting of a lipid bilayer, a solid-state membrane, and a liquid film material, and having a protein nanopore with a plurality of positively charged residues on an inner surface of the protein nanopore, wherein each of the plurality of positively charged residues includes a cysteine residue functionalized with maleimide;
- and
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells.
2 more elements
A nanopore sensor device, comprising:
- one or more cis wells;
- a cis electrode;
- a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a membrane selected from the group consisting of a lipid bilayer, a solid-state membrane, and a liquid film material, and having a nanopore;
- a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells;
- a first concentration of an electrolyte within the one or more cis wells;
- and
- a second concentration of the electrolyte within the trans wells, wherein the first concentration is higher than the second concentration and wherein a ratio of the first concentration to the second concentration ranges from about 10:1 to about 3:1;
- a stimulus source coupled to each of the plurality of trans electrodes either individually or via multiplexing, wherein the stimulus source is to cause current to flow through the nanopore;
- and
- a controller coupled to the stimulus source, the controller configured to individually/selectively address the plurality of trans electrodes to cause an ionic current through the nanopore of an addressed trans electrode of the plurality of trans wells;
- wherein the ionic current comprises an amount of anions of the electrolyte translocating through the nanopore to the addressed trans well that is higher than an amount of cations of the electrolyte translocating through the nanopore from the addressed trans well.
8 more elements
Dependencies are inferred from the claim text.