Split prism silicon-based liquid immersion microchannel measuring device and method

A split prism silicon-based immersion microchannel measuring device comprising:

• a microchannel structure including a support and one or more microchannels formed in the support and each having a sample detection layer with a fixed bioadhesive material for detecting a sample;
• a sample injection unit configured to inject a buffer solution containing the sample into the one or more microchannels;
• a prism unit having a first prism and a second prism and formed by connecting a vertical surface of the first prism and a vertical surface of the second prism;
• a blocking part disposed in a portion where the first prism and the second prism are connected to each other, the blocking part being configured to block an optical path;
• a polarized light generating unit configured to generate polarized light;
• and
• a polarized light detecting unit configured to detect a polarization change of reflected light,
• wherein the polarized light defines incident light that passes through the first prism and enters a first prism-buffer solution interface on which the first prism and the buffer solution adjoin each other,
• wherein a part of the incident light defines a first reflected light that passes through the first prism-buffer solution interface, is reflected by the sample detection layer, and passes through a second prism-buffer solution interface on which the second prism and the buffer solution adjoin each other,
• wherein another part of the incident light defines second reflected light that is reflected by the first prism-buffer solution interface, and
• wherein the second reflected light is absorbed, scattered, or reflected by the blocking part.