Automated sample preparation for spent media analysis
AssigneeIDEX Health & Science LLC
InventorsQuan Liu, John Barnacle, Eric Beemer
Life of this patent
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Abstract
A method for processing a sample of a cell culture composition includes diluting the sample followed by filtering the diluted sample, wherein the dilution may be at least 10 parts by volume of a diluent to one part sample, and the filter may be a porous membrane with a molecular weight cutoff of less than 20,000 Daltons. The dilution may be carried out at a multiple-port dilution valve having a first condition and a second condition. The sample may be mixed with the diluent at a predetermined dilution ratio when the dilution valve is in the second condition.
Claims13
A method for processing a cell culture composition of cells and spent media, the method comprising:
- (a) obtaining a sample of the cell culture composition from a source;
- (b) providing a processing apparatus having:
- (i) a dilution module for mixing the sample of the cell culture composition with a diluent to form a dilution composition, wherein the dilution module is operable in at least two conditions, with a first load condition loading the cell culture composition into a sample loading chamber, and a second inject condition combining the cell culture composition from the sample loading chamber with the diluent to form the dilution composition;
- (ii) a filtration module having a filter for filtering the dilution composition into a filtrate and a retentate;
- (iii) a sample collector for distinguishing a filtrate sample from the filtrate, wherein the processing apparatus defines a first fluidic pathway from the dilution module, through the filtration module, and to the sample collector;
- (c) motivating the dilution composition through the first fluidic pathway;
- and
- (d) operating the sample collector to:
- (i) begin distinguishing the filtrate sample from the filtrate at a first steady state time point when a concentration of the sample in the filtrate at the sample collector has increased to a first steady state concentration;
- and
- (ii) subsequent to step (d) (i), cease distinguishing the filtrate sample from the filtrate by no later than a second steady state time point when the concentration of the sample in the filtrate at the sample collector has decreased from the first steady state concentration by a predetermined threshold value.
Dependencies are inferred from the claim text.