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US 12,624,476 B2Granted
Chemically encoded spatially addressed library screening platforms
AssigneeCity of Hope
InventorsJacob Berlin, Gregory Copeland, Kathleen Elison, Hurik Muradyan
Life of this patent
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Abstract
Provided herein are encoded split pool libraries useful, inter alia, for forming highly diverse and dense arrays of ligand domains for screening and detection of a variety of ligand binder molecules. Also provided herein are methods for forming, decoding, binding to, and selectively cleaving the highly diverse and dense arrays of ligand domains.
Claims44
A method comprising:
- (a) providing a microparticle comprising a plurality of library molecule attachment points (A) on its surface and a plurality of encoding tag attachment points (B) on its surface,
- (b) attaching a first library molecule to one or more of the library molecule attachment points (A) through a first covalent linker,
- (c) attaching a first set of one or more encoding tags identifying said first library molecule to a fraction of said encoding tag attachment points (B) through a second covalent linker,
- (d) attaching a second library molecule to each first library molecule,
- (e) attaching a second set of one or more encoding tags identifying said second library molecule to another fraction of said encoding tag attachment points (B) through the second covalent linker,
- (f) optionally repeating steps (d) and (e) to attach additional library molecules and additional sets of one or more encoding tags identifying said additional library molecules to additional fractions of said encoding tag attachment points (B) through the second covalent linker, thereby forming a ligand domain attached to said microparticle, and
- (g) immobilizing the microparticle on a solid support,
- wherein said ligand domain is a small molecule or a peptide;
- wherein said second covalent linker comprises an acyl moiety or a sulfonamide moiety;
- and
- wherein said second covalent linker is cleavable under a condition that does not cleave said first covalent linker.
8 more elements
A method comprising:
- (a) providing a microparticle comprising a plurality of library molecule attachment points (A) on its surface and a plurality of encoding tag attachment points (B) on its surface,
- (b) attaching one or more library molecules to one or more of said library molecule attachment points (A) through a first covalent linker,
- (c) attaching one or more encoding tags identifying said one or more library molecules to a fraction of said encoding tag attachment points (B) through a second covalent linker, thereby forming a ligand domain attached to said microparticle, and
- (d) immobilizing said microparticle on a solid support,
- wherein said ligand domain is a small molecule or a peptide;
- wherein said second covalent linker comprises an acyl moiety or a sulfonamide moiety;
- and
- wherein said second covalent linker is cleavable under a condition that does not cleave said first covalent linker.
5 more elements
Dependencies are inferred from the claim text.