Single input multiplex decision systems and methods of using the same

A system comprising:

• a first expression vector comprising a first promoter sequence operably linked to a sequence encoding a first regulatory protein, wherein said first promoter is responsive to an input signal, and further comprising a second promoter sequence operably linked to a sequence encoding a second regulatory protein, wherein said second promoter is responsive to said first regulatory protein, and wherein said first expression vector is a low copy plasmid;
• a second expression vector comprising a third promoter sequence operably linked to a sequence encoding a first output protein, wherein said third promoter is responsive to said second regulatory protein and wherein said sequence encoding said first output protein further comprises a degradation tag, and further comprising a first copy of a fourth promoter sequence located between said third promoter and said sequence encoding said first output protein, wherein said first copy of said fourth promoter sequence transcribes in a direction opposite to said third promoter sequence, and further comprising a fifth promoter sequence operably linked to a sequence encoding said first output protein, wherein said fifth promoter is responsive to said first regulatory protein, and wherein said second expression vector is a high copy plasmid;
• and
• a third expression vector comprising a second copy of said fourth promoter operably linked to a sequence encoding a second output protein, and wherein said third expression vector is a medium copy plasmid.