Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof

A vector comprising one or more viral genes wherein each of the viral genes comprises a deletion to form a detective interfering gene (DIG), said vector expresses one or more nucleic acid molecules that interfere with expression of one or more wild-type viral genes that do not comprise the deletion, the DIG consists of one or more defective genes, that express detective interfering RNAs without any full-length viral RNA and do not generate self-replicable reassortants, wherein

• the vector further comprises a dual-functional peptide comprising an HIV-1 Tat Peptide (TAT) and a cationic peptide, comprising SEQ ID NO:
• 4 (P1);
• the DIG is a defective viral polymerase gene;
• the viral polymerase genes are PB2, PB1 and PA;
• the DIG comprises defective PB2, PB1 and PA;
• and
• the DIG comprises SEQ ID NO:
• 38 (DI-PA), SEQ ID NO:
• 39 (DI-PB1) and SEQ ID NO:
• 40 (DI-PB2),
• wherein the deletion is about 50-100 base pair, 100-150 base pair, 150-200 base pair, 200-250 base pair, 250-300 base pair, 300-350 base pair, 350-400 base pair, 400-450 base pair, 450-500 base pair, 500-550 base pair, 550-600 base pair, 600-650 base pair, 650-700 base pair, 700-750 base pair, 750-800 base pair, 800-850 base pair, 850-900 base pair, 900-950 base pair, 950-1000 base pair, 1000-1200 base pair, 1200-1500 base pair, 1500-1800 base pair, or 1800-2100 base pair in length.