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US 12,624,367 B2Granted
AAV6 variants
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsMavis Agbandje-McKenna, Antonette D. Bennett, Robert McKenna
Life of this patent
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Abstract
This disclosure relates to variant AAV6 particles engineered to escape host neutralizing antibodies but retain or improve transduction efficiency, and their use as gene delivery vehicles.
Claims19
A method comprising:
- administering to a subject a recombinant AAV6 (rAAV6) particle comprising an AAV6 capsid protein having a single substitution consisting of a substituted amino acid at position 531, wherein the substituted amino acid at position 531 is glutamic acid (E), aspartic acid (D), histidine (H), tyrosine (Y), methionine (M), or leucine (L), wherein the rAAV6 particle comprises a gene of interest, wherein the subject is seropositive for an adeno-associated virus (AAV) capsid protein of serotype 6 (AAV6) antigen comprising lysine 531, and wherein the subject is not seropositive for the capsid protein comprising the substituted amino acid.
A method comprising:
- administering to a subject a subsequent rAAV6 particle, wherein the subsequent rAAV6 particle comprises an AAV6 capsid protein having a single substitution consisting of a substituted amino acid at position 531, wherein the substituted amino acid at position 531 is glutamic acid (E), aspartic acid (D), histidine (H), tyrosine (Y), methionine (M), or leucine (L),
- wherein the subject had previously received a recombinant AAV (rAAV) particle comprising an AAV6 capsid protein that comprises lysine at position 531,
- wherein the subsequent rAAV6 particle comprises a gene of interest, wherein the subject is seropositive for an adeno-associated virus (AAV) capsid protein of serotype 6 (AAV6) antigen comprising lysine 531 and the subject is not seropositive for the capsid protein comprising the substituted amino acid.
A recombinant AAV6 (rAAV6) capsid protein comprising one of the following amino acids at position 531 according to SEQ ID NO:
- 1:
- histidine (H), methionine (M), or leucine (L).
Dependencies are inferred from the claim text.