Fusion proteins for DNA base editing
AssigneeSyngenta Crop Protection AG
InventorsJianping Xu, Jiang Li
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Abstract
The present invention relates to methods and compositions for modifying a target site in the genome of a cell. Fusion proteins including one or more DNA binding domains and one or more heterologous domains, such as DNA modifying domains, connected by improved linker sequences are provided. Codon optimized polynucleotides encoding fusion proteins including one or more DNA binding domains and one or more heterologous domains connected by improved linker sequences are provided.
Claims1
A fusion protein comprising from the N-terminus to the C-terminus a heterologous TadA deaminase domain, a first linker sequence, and a Type V CRISPR-Cas protein, wherein the first linker sequence comprises the sequence GGGGS at least six times (SEQ ID NO:
- 11), wherein the Type V CRISPR-Cas protein is a catalytically inactive Cas12a, wherein the fusion protein comprises SEQ ID NO:
- 81.
Dependencies are inferred from the claim text.