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US 12,624,352 B2Granted
DNA-responsive hydrogels
AssigneesMassachusetts Institute of Technology, President and Fellows of Harvard College
InventorsJames J. Collins, Helena de Puig Guixe, Luis Ruben Soenksen Martinez, Max English, Raphael Gayet, Nicolaas Angenent-Mari, Angelo S. Mao, Peter Q. Nguyen
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Abstract
Disclosed herein are hydrogels comprising a polynucleotide-based structural component. Methods of altering a property of a hydrogel based on user-defined nucleic acid input sequences are also disclosed. In addition, various applications are described that utilize these hydrogels and methods.
Claims5
A composition comprising:
- (i) a hydrogel comprising a plurality of structural components, wherein the plurality of structural components comprise polyethylene glycol (PEG) covalently bound to one or more DNA molecules, wherein the one or more DNA molecules comprise a single-stranded region;
- and
- (ii) a Cas12a protein, a guide RNA corresponding to the Cas12a protein, and a double-stranded DNA trigger molecule, wherein the guide RNA comprises a spacer region that is at least 85% complementary to a target sequence of the double-stranded DNA trigger molecule,
- wherein the Cas12a protein is capable of cleaving the single-stranded region of the one or more DNA molecules in the hydrogel following cleavage of the double-stranded DNA trigger molecule.
1 more element
Dependencies are inferred from the claim text.