Multilayer culture vessel

A multilayer culture vessel capable of culturing cells in a plurality of culture layers, the multilayer culture vessel comprising:

• a housing including a bottom plate, a top plate facing the bottom plate, and a plurality of side walls, which includes at least a first side wall and a second side wall opposite to the first side wall, connecting the bottom plate and the top plate;
• a boundary portion that divides an internal space of the housing into a culture space on one side of the boundary portion and a buffer space on the other side of the boundary portion in a first direction parallel to the bottom plate;
• at least one intermediate plate extending along the direction parallel to the bottom plate at least in the culture space and configured to divide the culture space into the plurality of culture layers;
• a plurality of wall portions at the boundary portion, the plurality of wall portions including one extending from the bottom plate in a direction toward the top plate and one extending from each of the at least one intermediate plate in a direction toward the top plate;
• a plurality of communication portions formed at the boundary portion to bring the culture layers into communication with the buffer space;
• a liquid supply/drainage port formed on the top plate of the housing at a location facing the buffer space;
• and
• an exhaust port that is formed, separately from the liquid supply/drainage port, on the top plate at a location facing the culture space,
• wherein the liquid supply/drainage port is arranged adjacent to the first side wall and the exhaust port is arranged adjacent to the second side wall, and
• wherein the first side wall and the second side wall are opposite to each other in a second direction perpendicular to the first direction.