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US 12,624,065 B2Granted
Use of neutral pH mobile phases in reversed phase chromatography of acidic peptides
AssigneeWaters Technologies Corporation
InventorsSamantha Ippoliti, Ying Qing Yu
Life of this patent
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Abstract
The present disclosure discusses a method of separating and/or purifying acidic peptides by the use of a mobile phase having a pH greater than or about equal to the isoelectric point of one or more of the metal oxides in the flow path.
Claims12
A method of separating sialylated O-glycopeptides, the method comprising:
- injecting a sample comprising a plurality of O-sialylated glycopeptides into a chromatographic system, the chromatographic system comprising a flow path, wherein at least a portion of the flow path comprises one or more metal oxides, wherein the O-sialylated glycopeptides have three or more sialylation sites per peptide;
- and
- flowing the sample through the chromatographic system under reverse phase chromatography conditions to separate the plurality of O-sialylated glycopeptides using a mobile phase having a pH of about 6.5 to about 8.5.
Dependencies are inferred from the claim text.