Systems and methods for particle sorting with automated adjustment of operational parameters

A method for calibration of particle sorting in a fluid stream, comprising:

• delivering a sequence of two or more sortable units from a fluid stream to an inspection zone using a particle delivery device;
• interrogating the two or more sortable units using an electromagnetic radiation source at the inspection zone;
• monitoring non-targeted sortable units that are located adjacent in sequence to targeted sortable units that are predicted to include one or more particles having a predetermined characteristic of interest in the sequence of sortable units using a monitoring system;
• sorting, using a sorter downstream of the electromagnetic radiation source, the adjacent non-targeted sortable units to a single output pathway monitored by the monitoring system using a sort logic, the single output pathway associated with a flow of the adjacent non-targeted sortable units;
• directing the targeted sortable units away from the single output pathway;
• interrogating, by the monitoring system, the adjacent non-targeted sortable units;
• and
• adjusting an operational parameter of the sort logic based upon a result of the interrogation of the adjacent non-targeted sortable units by the monitoring system.

A non-transitory computer-readable medium holding computing device-executable instructions for calibrating particle sorting in a fluid stream, the instructions when executed causing at least one computing device to:

• deliver a sequence of two or more sortable units from a fluid stream to an inspection zone using a particle delivery device operatively connected to the at least one computing device;
• interrogate the two or more sortable units using an electromagnetic radiation source at the inspection zone;
• monitor non-targeted sortable units that are located adjacent in sequence to targeted sortable units that are predicted to include one or more particles having a predetermined characteristic of interest in the sequence of sortable units using a monitoring system;
• sort, using a sorter downstream of the electromagnetic radiation source, the adjacent non-targeted sortable units to a single output pathway monitored by the monitoring system using a sort logic, the single output pathway associated with a flow of the adjacent non-targeted sortable units;
• direct the targeted sortable units away from the single output pathway;
• interrogate, by the monitoring system, the adjacent non-targeted sortable units;
• and
• adjust an operational parameter of the sort logic based upon a result of the interrogation of the adjacent non-targeted sortable units by the monitoring system.