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US 12,623,002 B2Granted
Fast automated approach for the derivation of acellular extracellular matrix scaffolds from tissues
AssigneeNorth Carolina State University
InventorsDonald O. Freytes, Camilo A. Mora-Navarro, Andreea Badileanu
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Abstract
Provided herein are systems, devices and methods to automate and optimize the decellularization process of representative tissues, such as soft tissues, for extracellular matrix (ECM)-based scaffold and biomaterial production. The automated decellularization processes and devices significantly reduce the exposure time to reagents, minimize lot-to-lot variability, and largely preserve the native composition of the ECM from the decellularized tissue or species.
Claims33
An automated tissue decellularization system, the system comprising:
- a bioreactor;
- a filtration device, optionally a lateral inline filtration device;
- a stirring mechanism;
- a temperature-controlled system for temperature adjustment and control of the bioreactor, and
- a fluid control system comprising a dosing system and/or one or more dosing pumps configured to automatically supply one or more reagents to the bioreactor, and/or to apply a feedback for in situ solubilization of ECM for downstream scaffold production, and/or remove waste from the bioreactor,
- wherein the automated decellularization bioreactor system is configured to decellularize a tissue, optionally a soft tissue, to produce an extracellular matrix (ECM) scaffold and/or to produce solubilized downstream ECM-products.
3 more elements
Dependencies are inferred from the claim text.