PCL patch tissue regeneration scaffold and method for manufacturing same
AssigneeAjou University Industry-Academic Cooperation Foundation
InventorsYun-Hoon Choung, Jong Hoon Chung, Hoon Seonwoo, Kyoung-Je Jang, Beomyong Shin
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Abstract
Disclosed is a method of treating a chronic tympanic perforation with a PCL patch tissue regeneration scaffold comprising preparing solution by adding polycaprolactone (PCL) and an acid to an organic solvent; preparing an electrospinning solution by adding a growth factor to the solution and stirring; collecting nanofibers arranged in a spindle shape on a collector by connecting the electrospinning solution prepared to a syringe pump and operating the electrospinning device; and administering the PCL patch tissue regeneration scaffold to a subject.
Claims2
A method of treating a chronic tympanic membrane perforation in a subject, comprising:
- preparing a spindle-shaped polycaprolactone (PCL) patch tissue regeneration scaffold by:
- preparing an electrospinning solution by adding the PCL and acetic acid to an organic solvent and stirring, wherein a concentration of the PCL in the organic solvent is 70 to 90 mg/ml;
- adding epithelial growth factor (EGF)
- and insulin growth factor binding protein (IGFBP) to the electrospinning solution and stirring at a temperature between 0° C. and 10° C., wherein the electrospinning solution comprises 0.16 to 16 μg/ml of the EGF and 0.16 to 16 μg/mL of the IGFBP;
- and collecting nanofibers on a cylindrical collector housing a single centrally located needle with a sharp end by electrospinning the electrospinning solution through a syringe pump at a flow rate of 0.2 to 3.0 μL/hr while applying 3 voltage of 18 kV across a distance of 10 cm between the single centrally located needle and the cylindrical collector, wherein the collected nanofibers are arranged in a radially aligned pattern converging from a periphery toward the single centrally located needle, thereby forming the spindle-shaped PCL patch tissue regeneration scaffold;
- and applying the spindle-shaped PCL patch tissue regeneration scaffold to the subject's tympanic membrane perforation of the subject without surgery, whereby the tympanic membrane at the chronic tympanie perforation is regenerated in the subject using the spindle-shaped PCL patch tissue regeneration scaffold loaded with the EGF and the IGFBP.
A method of treating a chronic tympanic membrane perforation in a subject, comprising:
- preparing a spindle-shaped PCL-PEG patch tissue regeneration scaffold by:
- preparing an electrospinning solution by adding polycaprolactone (PCL), polyethylene glycol (PEG), and acetic acid to an organic solvent and stirring to dissolve the polymers, wherein the electrospinning solution comprises PCL at a concentration of 100 to 160 mg/ml and PEG at a concentration of 40 to 100 mg/ml;
- adding epithelial growth factor (EGF) and insulin growth factor binding protein (IGFBP) to the electrospinning solution and stirring at a temperature between 0° C. and 10° C., wherein the electrospinning solution comprises 0.16 to 16 μg/ml of the EGF and 0.16 to 16 μg/ml of the IGFBP;
- and
- collecting nanofibers on a cylindrical collector housing a single centrally located needle with a sharp end by electrospinning the electrospinning solution through a syringe pump at a flow rate of 0.2 to 3.0 μL/hr while applying a voltage of 18 k kV across a distance of 10 cm between the single centrally located needle and the cylindrical collector, wherein the collected nanofibers are arranged in a radially aligned pattern converging from a periphery toward the single centrally located needle, thereby forming the spindle-shaped PCL-PEG patch tissue regeneration scaffold;
- and
- applying the spindle-shaped PCL-PEG patch tissue regeneration scaffold to the tympanic membrane perforation of the subject without surgery;
- whereby the tympanic membrane perforation is regenerated in the subject using the spindle-shaped PCL-PEG patch tissue regeneration scaffold loaded with the EGF and the IGFBP.
Dependencies are inferred from the claim text.