Pharmaceutical composition and use thereof

A pharmaceutical composition comprising:

• i) a hepatitis B surface antigen, an active fragment of the antigen, a variant of the antigen, or a mixture of at least two of them;
• ii) a hepatitis B core antigen, an active fragment of the antigen, a variant of the antigen, or a mixture of at least two of them;
• and
• iii) an immunostimulatory composition comprising a saponin and a CpG oligodeoxynucleotide, or consisting of an adjuvant comprising a saponin and a CpG oligodeoxynucleotide, wherein the sequence of the CpG oligodeoxynucleotide is any one selected from:
• CpG T1:
• TCG TTC GTT CGT TCG TTC GTT (SEQ ID NO:
• 6);
• CpG T2:
• TCG TTC GTT CGT TCG TTC GTT CGT T (SEQ ID NO:
• 7);
• and CpG T3:
• TCG TCG TCG TCG TCG TCG TCG (SEQ ID NO:
• 8), and the saponin is QS-21.