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US 12,622,878 B2Granted
Cationic lipid having cystine skeleton
AssigneesNof Corporation, National University Corporation Chiba University
InventorsShinya Tamagawa, Kota Tange, Yuta Nakai, Masamichi Tamada, Hidetaka Akita, Hiroki Tanaka, Yu Sakurai
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Abstract
The present invention provides, a cationic lipid represented by the formula (1) (wherein each symbol is as defined in the specification), a lipid membrane structure using the aforementioned cationic lipid, a nucleic acid-introducing agent using the aforementioned cationic lipid, and a method for introducing a nucleic acid by using the aforementioned nucleic acid-introducing agent.
Claims16
A cationic lipid represented by the formula (1):
- wherein
- L1a and L1b are each independently an amide bond, a carbamate bond, or a urea bond,
- R1a and R1b are each independently an alkylene group having not more than 8 carbon atoms or an oxydialkylene group having not more than 8 carbon atoms,
- ka and kb are each independently 0 or 1,
- Xa and Xb are each independently a dialkylamino group having two alkyl groups each independently having 1 to 5 carbon atoms, or a 3- to 6-membered cyclic amino group,
- L4a and L4b are each independently an ester bond or an amide bond,
- R2a and R2b are each independently an alkylene group having not more than 8 carbon atoms or an oxydialkylene group having not more than 8 carbon atoms,
- L2a and L2b are each independently an ester bond, an amide bond, a carbamate bond, an ether bond, or a urea bond,
- ma and mb are each independently 0 or 1,
- R3a and R3b are each independently a divalent group derived from an aromatic compound having 3 to 16 carbon atoms and at least one aromatic ring, and optionally having
- a hetero atom,
- L3a and L3b are each independently an ester bond, an amide bond, a carbamate bond, an ether bond, or a urea bond,
- na and nb are each independently 0 or 1,
- R4a and R4b are each independently an aliphatic hydrocarbon group having 12 to 22 carbon atoms, or R5—CO—(CH2)p—,
- R5 is a residue of a liposoluble vitamin having a hydroxy group or a residue of a sterol derivative having a hydroxy group, and
- p is 2 or 3.
13 more elements
Dependencies are inferred from the claim text.