System for detection of breast cancer margin and method thereof

A multispectral imaging system for real time detection of breast cancer margin during a tumor resection surgery by combining terahertz and autofluorescence images, comprising;

• a sample holder mounted on a stepper motor, adapted to hold and move a tissue sample in Z-axis and X-axis directions to enable raster scan of the sample;
• an optical controller, configured to accommodate at least one laser source to provide an optical input;
• a housing, configured to accommodate optical components, wherein the optical components include:
• a terahertz module having a terahertz emitter antenna adapted to generate terahertz radiation from the received optical input, one or more lenses to guide the generated terahertz radiation to the sample and a terahertz detector to receive reflected terahertz signal from the sample;
• a fluorescence module configured to generate and pass fluorescence radiation through the sample and to receive emitted autofluorescence, wherein the fluorescence module comprises:
• an imaging module configured to provide at least one excitation wavelength in infrared/ultraviolet range using one or more multiwavelength excitation LED to illuminate the sample to emit autofluorescence;
• an image acquisition module having one or more emission filters of predetermined wavelength adapted to filter the emitted autofluorescence from the sample;
• a camera to detect the filtered autofluorescence and generate an image based on autofluorescence;
• an electronic control unit connected to the optical controller to supply power, wherein the electronic control unit is configured to provide bias to the terahertz emitter antenna and form terahertz image with data acquisition unit;
• and
• a microcontroller connected to the electronic control unit, configured to display or to combine terahertz and autofluorescence images to determine breast cancer margin.