High rebaudioside M stevia plant cultivars and methods of producing the same
AssigneesPureCircle USA Inc., Keygene N.V., The Coca-Cola Company
InventorsAvetik Markosyan, Seong Siang Ong, Runchun Jing, Tengfang Huang, Fayaz Khazi, Stephen Ezra Schauer, Indra Prakash, Alec Hayes
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Abstract
Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.
Claims5
A Stevia rebaudiana plant comprising at least one disrupted negative regulator gene in the rebaudioside A to rebaudioside M conversion pathway, and wherein the negative regulator gene is selected from the group consisting of SEQ ID NO:9 and SEQ ID NO:10.
A method of increasing the rebaudioside M content in a Stevia rebaudiana plant by inducing a disruption in at least one negative regulator gene function in the plant, wherein said negative regulator gene is selected from the group consisting of SEQ ID NO:9 and SEQ ID NO:10.
A method of producing a Stevia plant comprising the steps of:
- a) introducing a recombinant DNA construct comprising a nucleotide sequence comprising one or more sequences selected from the group consisting of SEQ ID NO:9 and SEQ ID NO:10 operably linked to at least one heterologous nucleotide sequence into a Stevia rebaudiana plant, wherein said heterologous nucleotide sequence is a promoter;
- b) growing a fertile, mature plant resulting from step a);
- and
- c) selecting a plant expressing the at least one heterologous nucleotide sequence in at least one plant tissue.
Dependencies are inferred from the claim text.