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US 12,618,857 B2Granted
Methods for detecting estrone by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsMildred M. Goldman, Richard E. Reitz
Life of this patent
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Abstract
Provided are methods for determining the amount of estrone in a sample using mass spectrometry. The methods generally involve ionizing estrone in a sample and detecting and quantifying the amount of the ion to determine the amount of estrone in the sample.
Claims8
A method for determining an amount of estrone in a human serum or plasma sample, the method comprising:
- (a) adding deuterated estrone to a human serum or plasma sample;
- (b) purifying the estrone and the deuterated estrone from the human serum or plasma sample by high performance liquid chromatography (HPLC);
- (c) ionizing the purified estrone and deuterated estrone to produce one or more of estrone ions and deuterated estrone ions detectable by mass spectrometry,
- wherein the deuterated estrone ions comprise an ion having a mass/charge ratio of 159.10±0.5;
- and
- (d) detecting the amount of the estrone ions by tandem mass spectrometry, wherein the amount of the estrone ions is correlated to the amount of estrone in the human serum or plasma sample.
3 more elements
Dependencies are inferred from the claim text.