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US 12,618,845 B2Granted
Methods and compositions for protein sequencing
AssigneeQuantum-Si Incorporated
InventorsBrian Reed, Jeremy Lackey, Haidong Huang
Life of this patent
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Abstract
Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
Claims30
A method of sequencing a polypeptide, the method comprising:
- a) contacting a single polypeptide molecule with a composition comprising one or more terminal amino acid recognition molecules;
- b) detecting signal pulses indicative of association of the one or more terminal amino acid recognition molecules with a terminus of the single polypeptide molecule;
- c) contacting the single polypeptide molecule with a composition comprising one or more cleaving reagents;
- and
- d) repeating (a)-(c) one or more times,
- wherein the detected signal pulses form a series of signal pulses that is indicative of a series of amino acids exposed at the terminus over time as a result of terminal amino acid cleavage by the one or more cleaving reagents, and
- wherein the single polypeptide molecule is immobilized to a surface through a linkage group comprising an oligonucleotide.
4 more elements
Dependencies are inferred from the claim text.