Testing of luminescence imaging apparatus with automatic detection of a testing device

A computer-readable medium storing instructions that when executed by a control unit of a luminescence imaging apparatus, cause the control unit:

• to acquire, with a photograph camera of an imaging head, a photograph image of a field of view representative of a reflected light being reflected by the field of view,
• to retrieve a descriptor of the testing device comprising an indication of a geometry of the testing device and of a position of the sites in the testing device, the indication of the geometry of the testing device comprising real-world coordinates of geometry indicators indicative of a position of the geometry indicators in the testing device,
• to find a position of the testing device in the photograph image according to the geometry of the testing device being indicated in the descriptor,
• to calculate a transformation between the real-world coordinates of the geometry indicators being indicated in the descriptor and corresponding image coordinates of the geometry indicators being found in the photograph image,
• to acquire, with a luminescence camera of the imaging head, a luminescence image of the field of view representative of a luminescence light being emitted by the luminescence substance of the sites in response to an excitation light thereof provided by an excitation light source of the imaging head,
• to determine a representation of the sites in the luminescence image according to an application of the transformation to the position of the sites in the testing device being indicated in the descriptor, and
• to test the luminescence imaging apparatus by verifying a specification of the luminescence light defined by the representation of the sites in the luminescence image.

A computer program product comprising a computer readable storage medium embodying a computer program being loadable into a working memory of a control unit of a luminescence imaging apparatus thereby configuring the control unit:

• to acquire, with a photograph camera of an imaging head, a photograph image of a field of view representative of a reflected light being reflected by the field of view,
• to retrieve a descriptor of the testing device comprising an indication of a geometry of the testing device and of a position of the sites in the testing device, the indication of the geometry of the testing device comprising real-world coordinates of geometry indicators indicative of a position of the geometry indicators in the testing device,
• to find a position of the testing device in the photograph image according to the geometry of the testing device being indicated in the descriptor,
• to calculate a transformation between the real-world coordinates of the geometry indicators being indicated in the descriptor and corresponding image coordinates of the geometry indicators being found in the photograph image,
• to acquire, with a luminescence camera of the imaging head, a luminescence image of the field of view representative of a luminescence light being emitted by the luminescence substance of the sites in response to an excitation light thereof provided by an excitation light source of the imaging head,
• to determine a representation of the sites in the luminescence image according to an application of the transformation to the position of the sites in the testing device being indicated in the descriptor, and
• to test the luminescence imaging apparatus by verifying a specification of the luminescence light defined by the representation of the sites in the luminescence image.

A luminescence imaging apparatus comprising a control unit configured:

• to acquire, with a photograph camera of an imaging head, a photograph image of a field of view representative of a reflected light being reflected by the field of view,
• to retrieve a descriptor of the testing device comprising an indication of a geometry of the testing device and of a position of the sites in the testing device, the indication of the geometry of the testing device comprising real-world coordinates of geometry indicators indicative of a position of the geometry indicators in the testing device,
• to find a position of the testing device in the photograph image according to the geometry of the testing device being indicated in the descriptor,
• to calculate a transformation between the real-world coordinates of the geometry indicators being indicated in the descriptor and corresponding image coordinates of the geometry indicators being found in the photograph image,
• to acquire, with a luminescence camera of the imaging head, a luminescence image of the field of view representative of a luminescence light being emitted by the luminescence substance of the sites in response to an excitation light thereof provided by an excitation light source of the imaging head,
• to determine a representation of the sites in the luminescence image according to an application of the transformation to the position of the sites in the testing device being indicated in the descriptor, and
• to test the luminescence imaging apparatus by verifying a specification of the luminescence light defined by the representation of the sites in the luminescence image.