Instrument and method for extracting and detecting nucleic acids
AssigneeJiaxing Accunome Biotechnology Co., Ltd.
InventorsXing Yang, Xianhua Wang, Yang Song, Jianxin Ye, Wei Zhang
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Abstract
Provided is a device for extracting and detecting nucleic acids, comprising one or more sample receiving modules, one or more lysing modules, one or more extraction module of nucleic acids, one or more amplification module of nucleic acids, and a detection module. Also provided is a molecular in vitro diagnostic instrument comprising the device for extracting and detecting nucleic acids and an automatic control system. Further provided is an in vitro diagnosis method by means of performing extraction and amplification of nucleic acids in a sample using the device for extracting and detecting nucleic acids.
Claims21
A device for extracting and detecting nucleic acids, comprising:
- one or more sample receiving modules, each sample receiving module comprises a chamber adapted to receive a sample or sample container;
- one or more lysing modules, each lysing module comprises a chamber adapted to receive a lysing reagent or a lysing kit;
- one or more nucleic acid extraction modules, each nucleic acid extraction module of nucleic acids comprises a chamber adapted to receive nucleic acid extraction reagents or a nucleic acid extraction kit;
- one or more nucleic acid amplification modules, each nucleic acid amplification module comprises a plurality of chambers adapted to receive nucleic acid amplification reagents or an amplification tube of a nucleic acid amplification kit,
- wherein the one or more nucleic acid amplification modules further comprise a capping mechanism to close a cap of the amplification chamber or amplification tube, the capping mechanism comprising a cap assembly and a motion assembly, wherein the cap assembly comprises a cap clamping plate and a stepping motor,
- wherein the stepping motor controls up and down movements of the cap clamping plate,
- wherein the motion assembly is configured to move back and forth in the horizontal direction,
- wherein the amplification chamber of the nucleic acid amplification module is connected to the motion assembly,
- wherein the capping mechanism is configured so that:
- when the amplification chamber or the amplification tube moves with the motion assembly to be close to the cap clamping plate, the stepping motor drives the cap clamping plate to move up, lifts the cap of the amplification chamber or the amplification tube, and flips the cap;
- and
- when the cap is lifted to an appropriate angle, the cap clamping plate no longer moves upward, the motion assembly continues to move toward the cap clamping plate, and then the stepping motor drives the clamping plate to move downward to press the cap;
- wherein the device further comprises a detection module.
Dependencies are inferred from the claim text.