Back to results
US 12,618,100 B2Granted
Rapid cellular lysis by reduction/oxidation reaction
AssigneeLuminex Corporation
InventorsEdward A. Sekinger, Megan Martinez, Mahima Pancholi, Kurt Hoffacker
Life of this patent
Estimating the term…
Abstract
Provided herein are methods for the rapid preparation of amplifiable nucleic acids from biological samples, which can be applied to various applications, such as, for example, point-of-care diagnostics, service laboratory diagnostics, and molecular biology applications. These methods can be performed in 15 minutes or less, and preferably in 5 minutes or less. For most applications, no further purification of nucleic acids is needed.
Claims15
A method for preparing amplifiable nucleic acids from a biological sample, the method comprising:
- (a) contacting the biological sample with a percarbonate salt, a nuclease suppressor, and a chelator to form a redox reaction composition;
- (b) incubating the redox reaction composition at a first temperature, wherein the first temperature is from 20° C. to 65° C., wherein the redox composition is incubated at the first temperature for 1 to 3 minutes;
- and
- (c) incubating the redox reaction composition at a second temperature, wherein the second temperature is from 60° C. to 100° C., wherein the redox reaction composition is incubated at the second temperature for 30 to 90 seconds,
- thereby obtaining amplifiable nucleic acids from the biological sample without purification of the nucleic acids following the redox reaction.
2 more elements
Dependencies are inferred from the claim text.