Medium for Bacillus cereus group detection
AssigneeShimadzu Diagnostics Corporation
InventorYusuke Koyanagi
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Abstract
A medium for Bacillus cereus group detection, which is favorable in growth of Bacillus cereus regardless of the temperature condition, and further is excellent in selectivity; and a method for detecting a Bacillus cereus group using the medium. The medium for Bacillus cereus group detection includes a phosphatidylinositol-specific phospholipase C substrate having a detectable chromogenic or fluorescent free radical; and trimethoprim. The medium further includes a β-lactam antibiotic. The medium further includes an antifungal agent. The method for detecting further includes inoculating a sample into the medium to culture the sample; and determining a detectable colony on the medium.
Claims3
A medium for Bacillus cereus group detection, comprising, as concentrations at the time of detection:
- from 0.001 to 10 g/l of a phosphatidylinositol-specific phospholipase C substrate having a detectable chromogenic or fluorescent free radical;
- from 0.01 to 500 mg/l of trimethoprim;
- from 0.001 to 100 mg/l of a cephem antibiotic or a monobactam antibiotic;
- and
- from 0.001 to 100 mg/l of a polyene antifungal agent,
- wherein the cephem antibiotic comprises ceftazidime, the monobactam antibiotic comprises aztreonam, and the polyene antifungal agent comprises amphotericin B,
- wherein the Bacillus cereus group is at least one bacterium selected from the group consisting of Bacillus cereus, Bacillus anthracis, Bacillus thuringiensis, Bacillus mycoides, Bacillus pseudomycoides, Bacillus weihenstephanensis, Bacillus cytotoxicus, and Bacillus toyonensis.
Dependencies are inferred from the claim text.