Solanaceous plant and solanaceous plant cell having resistance to tomato spotted wilt virus, and method for producing solanaceous plant

A method for producing a tomato spotted wilt virus resistant solanaceous plant, the method comprising:

• selecting the receptor-like kinase RLK gene having a cDNA sequence comprising the nucleotide sequence as set forth in SEQ ID NO:1;
• introducing a mutation into the selected gene in a genome of a solanaceous plant by genome editing techniques, wherein the mutation is introduced so that the RLK gene has a cDNA sequence comprising the nucleotide sequence as set forth in SEQ ID NO:
• 10 or SEQ ID NO:
• 11,
• wherein the introduced mutation is either a mutation inhibiting an expression of the mutated gene or a mutation making a protein encoded by the mutated gene to be non-functional for tomato spotted wilt virus, and wherein the protein which is non-functional for the virus is either a protein which cannot be used by the virus during infection and replication, or a protein which reduces the infection and replication of the virus, as compared to a control solanaceous plant without the introduction of the mutation;
• and
• selecting the solanaceous plant showing lower infection rate for the tomato spotted wilt virus, as compared to control solanaceous plant without the introduction of the mutation, wherein the infection rate is based on the presence or absence of tomato spotted wilt virus symptoms judged by observation, and/or the presence or absence of a gene of the tomato spotted wilt virus detected by PCR, on day 20 or more post tomato spotted wilt virus inoculation.