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AssigneesFondazione Edmund Mach, SciENZA Biotechnologies 4 B.V.
InventorsLisa Giacomelli, Tieme Zeilmaker, Claudio Moser, Jeroen Nicolaas Albert Maria Rouppe Van Der Voort
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Provided herein are powdery mildew-resistant grapevine plants and methods for providing powdery mildew resistance to susceptible grapevine plants. Specifically, provided herein are grapevine plants including in their genome an impaired Erysiphe necator resistance-conferring gene, wherein the corresponding not impaired Erysiphe necator resistance conferring resistance-conferring gene designated VvMLO13 encodes a protein including the amino acid sequence of SEQ ID No. 1, or proteins having 95% sequence identity therewith. The impairment results in an absence of a protein comprising the amino acid sequence of SEQ ID No. 1, or proteins having 95% sequence identity therewith, in the grapevine plant and wherein the grapevine plant is resistant to powdery mildew.
A powdery mildew-resistant grapevine plant comprising in its genome a mutation in a gene having the nucleotide sequence of SEQ ID No. 2, wherein the mutation results in the absence of a protein comprising the amino acid sequence of SEQ ID No. 1, or a protein having 95% sequence identity therewith, and confers resistance to Erysiphe necator in said grapevine plant.
A method for providing a powdery mildew-resistant grapevine plant, comprising mutating a gene having the nucleotide sequence of SEQ ID No. 2, wherein the mutation results in the absence of a protein comprising the amino acid sequence of SEQ ID No. 1, or a protein having 95% sequence identity therewith, in a powdery mildew-susceptible grapevine plant, thereby providing a grapevine plant that is resistant to powdery mildew.
Dependencies are inferred from the claim text.