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US 12,618,076 B2Granted
Methods of engineering platelets for targeting circulating tumor cells
AssigneeUniversity of Utah Research Foundation
InventorTara Deans
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Abstract
Disclosed herein are nucleic acid constructs that can be used to build genetic circuits for producing antibodies comprising split toxins. Also disclosed herein are methods of producing platelets comprising the antibodies. The platelets produced by the methods disclosed herein can be used to target circulating tumor cells.
Claims12
A nucleic acid construct comprising:
- a) a promoter operatively linked to a sequence encoding c-MYC, BMI1, and BCL-XL;
- and
- b) a sequence encoding an engineered antibody sequence, wherein the engineered antibody sequence comprises a split toxin sequence flanked by an intein sequence;
- wherein the sequence encoding c-MYC, BMI1, and BCL-XL is flanked by a first recombination site and a second recombination site;
- wherein the sequence encoding the engineered antibody sequence is out of frame from the promoter;
- wherein, when exposed to a recombinase under conditions suitable to catalyze a site-specific recombination event between the first recombination site and the second recombination site, the sequence encoding c-MYC, BMI1, and BCL-XL is excised and the sequence encoding the engineered antibody sequence is brought in frame with the promoter.
3 more elements
Dependencies are inferred from the claim text.