CD40 specific DNA aptamers as vaccine adjuvants
AssigneesBoard of Trustees of the University of Arkansas, The Texas A&M University System
InventorsAdil Sabr Al-Ogaili, Tieshan Jiang, Christine N. Vuong, Rohana Liyanage, Jackson O. Lay, Jr., Suresh Kumar Thallapuranam, Luc R. Berghman, Young Min Kwon, Billy Hargis
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Abstract
The present invention provides immunostimulatory nucleic acids that have an affinity to a specific target protein. The present invention also provides templates and methods for making and using the immunostimulatory nucleic acids. Further, methods for linking the immunostimulatory nucleic acids to antigens and using the resulting complexes to enhance an immune response are provided.
Claims18
An immunostimulatory nucleic acid comprising a protein-binding sequence having at least 95% identity to any one of SEQ ID NOs:
- 1-8, wherein the immunostimulatory nucleic acid binds to CD40.
An immunostimulatory nucleic acid comprising a plurality of protein-binding units;
- wherein each of the protein-binding units comprise a first protein-binding sequence, a second protein-binding sequence, and a spacer sequence therebetween;
- wherein the second protein-binding sequence is a different sequence than the first protein-binding sequence;
- wherein (a) the first protein-binding sequence has at least 95% identity to any one of SEQ ID NOs:
- 1-8, (b) the second protein-binding sequence has at least 95% identity to any one of SEQ ID NOs:
- 1-8, or (c) both the first protein-binding sequence and the second protein-binding sequence have at least 95% identity to any one of SEQ ID NOs:
- 1-8;
- and wherein the immunostimulatory nucleic acid binds to CD40.
A template for producing a plurality of protein-binding units comprising in order from 5′ to 3′:
- i) a 3′ end of a primer-binding sequence, ii) a reverse complement of a second protein-binding sequence, iii) a reverse complement of a spacer sequence, iv) a reverse complement of a first protein-binding sequence, and v) a 5′ end of a primer-binding sequence, wherein the 5′ end of the primer-binding sequence and the 3′ end of the primer-binding sequence form a primer-binding site when the template is circularized.
Dependencies are inferred from the claim text.