Modified enzyme and method of producing imidazole dipeptide using same
AssigneeAjinomoto Co., Inc.
InventorsMizuki Tamori, Uno Tagami, Hiroyuki Nozaki
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Abstract
Described herein is an enzyme useful for producing a target peptide, capable of producing the target peptide without requiring ATP. More specifically, provided herein is a modified enzyme includes an amino acid sequence such as a modified amino acid sequence including a mutation of one or more of the following amino acid residues: E81, I127, I136, T139, F140, G142, W143, I147, I181, I201, Q219, T229, M244, A249, P255, E256, I260, S293, N294, Y295, and I299 in an amino acid sequence of SEQ ID NO: 1, and having the following property (a) or (b) enhanced relative to an enzyme of the amino acid sequence of SEQ ID NO: 1: (a) imidazole dipeptide production activity; or (b) thermal stability.
Claims13
A modified enzyme comprising:
- (A) an amino acid sequence of SEQ ID NO. 1, wherein the amino acid sequence additionally comprises a mutation of an amino acid residue selected from the group consisting of E81, I127, I136, T139, F140, G142, W143, I147, I181, I201, Q219, T229, M244, A249, P255, E256, I260, S293, N294, Y295, I299, and combinations thereof;
- (B) the amino acid sequence of (A) comprising an additional mutation of substitution, deletion, insertion, and addition of one or more amino acid residues;
- or
- (C) an amino acid sequence having 90% or more identity to said amino acid sequence of (A) or (B);
- and
- wherein an imidazole dipeptide production activity or thermal stability is enhanced in the modified enzyme relative to an enzyme of the amino acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.