Reprogramming methods and cell culture platforms
AssigneeFate Therapeutics, Inc.
InventorsBahram Valamehr, Peter Flynn, Ramzey Abujarour, Megan Robinson
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Abstract
The invention provides compositions and methods for manufacturing pluripotent cells. In particular, the invention provides improved culture platforms for manufacturing pluripotent cells with ground state pluripotency. In various embodiments, the invention contemplates, in part, a composition comprising: (a) a Wnt pathway agonist; (b) a MEK inhibitor; and (c) a ROCK inhibitor. In certain embodiments, the composition further comprises bFGF or LIF.
Claims15
A culture system comprising a cell culture platform for manufacturing induced pluripotent stem cells (iPSCs), the cell culture platform comprising a first composition and a second composition, wherein:
- (i) the first composition comprises non-pluripotent cells in a first culture medium comprising a Wnt pathway agonist, a MEK inhibitor, a ROCK inhibitor, and a TGFβR inhibitor;
- (ii) the second composition comprises iPSCs in a second culture medium comprising:
- (a) a Wnt pathway agonist;
- (b) a MEK inhibitor;
- and
- (c) a ROCK inhibitor;
- and
- (iii) the second composition does not comprise a TGFβR inhibitor.
Dependencies are inferred from the claim text.