Multi-well plate and method for preparing same

A multi-well plate comprising

• a support, an upper surface of said support being at least partially covered with a continuous layer of a hydrogel which has a stiffness of from 0.05 kPa to 100 kPa, as measured by atomic force microscopy, said continuous layer of the hydrogel being in contact with a lower surface of a bottomless multi-well plate, wherein said support, said continuous layer of the hydrogel, and the said bottomless multi-well plate are adhered by an adhesive which extends from at least certain portions of the lower surface of the bottomless multi-well plate up to certain portions of the upper surface of said support by passing through the continuous layer of hydrogel, said continuous layer of the hydrogel being partitioned into compartments by means of the adhesive, a bottom of each well of the multi-well plate being entirely surrounded by said at least certain portions of the lower surface of the bottomless multi-well plate.