Protease-cleavable substrates and methods of use thereof
AssigneeCytomX Therapeutics, Inc.
InventorsOlga Vasiljeva, Michael B. Winter
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Abstract
Isolated polypeptides that include a cleavable moiety that is a substrate for at least one protease (e.g., MT-SP1 and/or an MMP) and isolated polypeptides that include a substrate that has a first cleavable moiety cleavable by a first protease and a second cleavable moiety cleavable by a second protease are disclosed. Activatable molecules including the isolated polypeptides are disclosed. Methods of making and using the isolated polypeptides and activatable molecules including the isolated polypeptides in a variety of therapeutic, diagnostic, and prophylactic applications are disclosed.
Claims30
An isolated polypeptide comprising a substrate, wherein the substrate comprises a sequence selected from the group consisting of SEQ ID NOs:
- 13, 163, 164, 177, 178, 191-193, 690, and 691, wherein the isolated polypeptide comprises a first linking peptide (LP1)N-terminal to the sequence, and a second linking peptide (LP2)C-terminal to the sequence.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 12, 13, 163, 164, 177, 178, 191-193, 690, and 691, wherein the isolated polypeptide is an activatable molecule and further comprises an active moiety (AM) that specifically binds a target.
An isolated polypeptide comprising a substrate, wherein the substrate comprises a sequence selected from the group consisting of SEQ ID NOs:
- 11, 120, 132, 138, 144, 222 and 223.
Dependencies are inferred from the claim text.