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US 12,616,776 B2Granted
Three-dimensional tissue body, method for producing same, and formation agent for three-dimensional tissue body
AssigneesToppan Printing Co., Ltd., Osaka University
InventorsMichiya Matsusaki, Shinji Irie, Shiro Kitano
Life of this patent
Estimating the term…
Abstract
A method may comprises bringing cells suspended in an aqueous medium into contact with a plurality of fragmented collagen pieces and, after the cells brought into contact with the plurality of fragmented collagen pieces and the plurality of fragmented collagen pieces are concentrated, culturing the cells brought into contact with the fragmented collagen pieces, with the plurality of fragmented collagen pieces, to form a three-dimensional tissue.
Claims17
A method, comprising:
- bringing cells into contact with a plurality of fragmented collagen pieces derived from exogenous collagen which is exogenous to the cells in an aqueous medium, the bringing of the cells including adding a dispersion of the plurality of fragmented collagen pieces to the aqueous medium containing the cells, or adding the cells to the aqueous medium containing a dispersion of the plurality of fragmented collagen pieces, resulting in a suspension of the cells and the plurality of fragmented collagen pieces in the aqueous medium;
- and
- culturing the cells with the plurality of fragmented collagen pieces, pieces to form a three-dimensional tissue, the culturing including standing the suspension of the cells and the plurality of fragmented collagen pieces to allow sedimentation, resulting in the plurality of fragmented collagen pieces being uniformly distributed among the cells, three-dimensionally throughout the three-dimensional tissue.
A method comprising:
- bringing cells into contact with a plurality of fragmented collagen pieces in an aqueous medium, the bringing of the cells including adding a dispersion of the plurality of fragmented collagen pieces to the aqueous medium containing the cells, or adding the cells to the aqueous medium containing a dispersion of the plurality of fragmented collagen pieces, resulting in a suspension of the cells and the plurality of fragmented collagen pieces in the aqueous medium;
- culturing the cells with the plurality of fragmented collagen pieces to form a three-dimensional tissue, the culturing including standing the suspension of the cells and the plurality of fragmented collagen pieces to allow sedimentation, resulting in the plurality of fragmented collagen pieces being uniformly distributed among the cells, three-dimensionally throughout the three-dimensional tissue.
Dependencies are inferred from the claim text.