Photosensitizing antibody-fluorophore conjugates
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsHisataka Kobayashi, Peter Choyke
Life of this patent
Estimating the term…
Abstract
Methods of killing cells are described. In particular examples, the method includes contacting a cell having a cell surface protein with a therapeutically effective amount of an antibody-IR700 molecule, wherein the antibody specifically binds to the cell surface protein, such as a tumor-specific antigen on the surface of a tumor cell. The cell is subsequently irradiated, such as at a wavelength of 660 to 740 nm at a dose of at least 1 J cm−2. The cell is also contacted with one or more therapeutic agents (such as an anti-cancer agent), for example about 0 to 8 hours after irradiating the cell, thereby killing the cell. Also provided are methods of imaging cell killing in real time, using fluorescence lifetime imaging.
Claims21
A method of treating a tumor and detecting margins of the tumor during a surgical procedure, the method comprising:
- a) administering a therapeutically effective amount of an antibody-IR700 conjugate to a subject having a tumor, wherein the antibody binds to a cell surface protein on the tumor;
- b) irradiating the tumor at a wavelength between 660-740 nm and at a dose of at least 1 J cm−2, thereby treating the tumor in the subject;
- and
- c) detecting fluorescence of the antibody-IR700 conjugate following the irradiating step to detect the tumor margins.
A method of treating a tumor in a subject and monitoring the treatment of the tumor, the method comprising:
- a) administering a therapeutically effective amount of an antibody-IR700 conjugate to the subject, wherein the antibody specifically binds to a cell surface protein on the tumor;
- b) irradiating the tumor at a wavelength between 660-740 nm and at a dose of at least 1 J cm−2, thereby treating the tumor in the subject;
- and
- c) detecting fluorescence of the antibody-IR700 conjugate following the irradiating step to monitor the course of treatment.
Dependencies are inferred from the claim text.