Influenza virus backbone
AssigneeFluGen, Inc.
InventorsYasuko Hatta, Michael J. Moser, Pamuk Bilsel
Life of this patent
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Abstract
The invention provides an influenza virus that demonstrates enhanced growth in Vero cells. The influenza virus includes PB1, PB2, PA, NP, and NS gene segments encoding proteins having amino acid sequences with selected amino acids. Optionally, at least one of the PB1, PB2, and PA gene segments includes a cytosine to uracil promoter mutation at nucleotide position 4. The invention also provides a pharmaceutical formulation containing the influenza virus, as well as a method of eliciting an immune response in a mammal by administering the influenza virus to the mammal, and a method for generating the influenza virus.
Claims33
An influenza virus comprising PB1, PB2, PA, NP, and NS gene segments, wherein
- (a) the PB1 gene segment encodes a PB1 protein having the amino acid sequence of SEQ ID NO:
- 7, the PB2 gene segment encodes a PB2 protein having the amino acid sequence of SEQ ID NO:
- 15, the PA gene segment encodes a PA protein having the amino acid sequence of SEQ ID NO:
- 17, the NP gene segment encodes an NP protein having the amino acid sequence of SEQ ID NO:
- 6, and the NS gene segment encodes an NS1 protein having the amino acid sequence of SEQ ID NO:
- 19, or
- (b) the PB1 gene segment encodes a PB1 protein having the amino acid sequence of SEQ ID NO:
- 9, the PB2 gene segment encodes a PB2 protein having the amino acid sequence of SEQ ID NO:
- 10, the PA gene segment encodes a PA protein having the amino acid sequence of SEQ ID NO:
- 17, the NP gene segment encodes an NP protein having the amino acid sequence of SEQ ID NO:
- 8, and the NS gene segment encodes an NS1 protein having the amino acid sequence of SEQ ID NO:
- 19.
Dependencies are inferred from the claim text.