Cell preparation for extemporaneous use, useful for healing and rejuvenation in vivo
AssigneeRegenLab USA LLC
InventorsAntoine Turzi, Donald Du Toit
Life of this patent
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Abstract
The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell composition preparations for use in tissue regeneration and bone regeneration and pain reduction.
Claims26
A non-activated cosmetic composition comprising:
- a platelet concentrate wherein the platelet concentrate is obtained by a one-step centrifugation process that separates erythrocytes while retaining plasma fractions enriched in functionally active platelets, the process comprising:
- introducing whole blood to a separator tube comprising an anticoagulant composition and a thixotropic gel;
- centrifuging the separator tube at a force between about 1500 g and about 2000 g to separate plasma from red blood cells of the whole blood;
- collecting a plasma fraction enriched in platelets without removal of platelet poor plasma resulting in retention of plasma proteins;
- and
- re-suspending platelets in remaining plasma after optionally discarding a portion of platelet poor plasma supernatant;
- wherein the platelet concentrate:
- concentrates plasma in leucocytes, thrombocytes, and adhesion proteins;
- comprises at least 300 billion platelets per liter;
- exhibits 2-4 times platelet and growth factor levels compared to a natural blood clot;
- maintains fibrin and fibrinogen levels comparable to the natural blood clot;
- and maintains stability of platelet-derived growth factors for at least 48 hours post-preparation at room temperature.
A non-activated cosmetic composition comprising:
- a platelet concentrate prepared by through a single centrifugation step that minimizes red blood cell contamination while retaining a plasma fraction enriched in platelets, without requiring cryopreservation or exogeneous activation prior to application:
- wherein the platelet concentrate:
- contains greater than 300 billion platelets per liter,
- contains white blood cells at a concentration of at least 700 billion cells per liter,
- contains fibrinogen at a concentration of least 3 mg per liter,
- contains erythrocytes at a concentration of less than 0.6×1012 cells per liter, and maintains stability of platelet-derived growth factor functionality for at least 48 hours post-preparation at room temperature.
Dependencies are inferred from the claim text.