Lipid particles for nucleic acid delivery and clinical applications of same
AssigneeRamot at Tel-Aviv University Ltd.
InventorsDan Peer, Daniel Rosenblum, Anna Gutkin
Life of this patent
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Abstract
Lipid particles for nucleic acid delivery and clinical applications of same are provided. Accordingly there is provided a lipid particle comprising a cationic lipid encapsulating a nucleic acid sequence, wherein said nucleic acid sequence encodes a protein having a length of at least 500 amino acids, the cationic lipid being represented by Formula I, as defined in the specification.
Claims12
A lipid particle comprising a cationic lipid encapsulating a nucleic acid sequence, wherein said nucleic acid sequence encodes a protein having a length of at least 500 amino acids, the cationic lipid being represented by Formula I:
- wherein:
- m is 0 or 1
- A1 and A2 are each independently a saturated or unsaturated linear, non-branched, alkylene chain, of at least 8 carbon atoms in length;
- L1 is a first linking group which an alkylene of 1 to 4 carbon atoms in length;
- X is —O—C(═O)—or —NH—C(═O);
- L2 is a second linking group which is an alkylene of 1 to 4 carbon atoms in length;
- and
- R1 and R2 are each independently hydrogen, alkyl or cycloalkyl, or, alternatively, R1 and R2 form together with the nitrogen to which they are attached a heteroalicyclic ring,
- provided that when X is —O—C(═O)—, m is 1.
A method of preparing a lipid particle for delivery of a nucleic acid sequence, the method comprising encapsulating a nucleic acid sequence in a lipid particle comprising a cationic lipid represented by Formula I:
- wherein:
- m is 0 or 1
- A1 and A2 are each independently a saturated or unsaturated linear, non-branched, alkylene chain, of at least 8 carbon atoms in length;
- L1 is a first linking group which an alkylene of 1 to 4 carbon atoms in length;
- X is —O—C(—O)—or —NH—C(═O);
- L2 is a second linking group which is an alkylene of 1 to 4 carbon atoms in length;
- and
- R1 and R2 are each independently hydrogen, alkyl or cycloalkyl, or, alternatively, R1 and R2 form together with the nitrogen to which they are attached a heteroalicyclic ring,
- provided that when X is —O—C(═O)—, m is 1,
- wherein said nucleic acid sequence encodes a protein having a length of at least 500 amino acids.
Dependencies are inferred from the claim text.