Lenses for treating ocular diseases and preparation method thereof
AssigneeGuangdong ProCapZoom Biosciences Co., Ltd.
InventorsHaoyu Zeng, Zhenbo Shen, Yuanyue Liu, Tian Guan, Jiexing Chen
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Abstract
The disclosure provides lenses for treating ocular diseases, including a bowl-shaped silicone hydrogel carrier and umbilical cord mesenchymal stem cells loaded on the carrier; the bowl-shaped silicone hydrogel carrier is polymerized from an organosilicone monomer and a hydrophilic monomer under the action of a cross-linking agent and an initiator; the organosilicone monomer is polymerized from a hydrogenated silicone oil and allyl methacrylate under the action of a polymerization inhibitor and a catalyst; and the umbilical cord mesenchymal stem cells are treated with irradiation.
Claims15
Lenses for treating ocular diseases, comprising a bowl-shaped silicone hydrogel carrier and umbilical cord mesenchymal stem cells loaded on the carrier;
- wherein the bowl-shaped silicone hydrogel carrier is polymerized from an organosilicone monomer and a hydrophilic monomer under an action of a cross-linking agent and an initiator;
- the organosilicone monomer is polymerized from a hydrogenated silicone oil and allyl methacrylate under the action of a polymerization inhibitor and a catalyst;
- and the umbilical cord mesenchymal stem cells are treated with irradiation.
A preparation method of lenses for treating ocular diseases, comprising steps as follows:
- 1) culturing and expanding umbilical cord mesenchymal stem cells with a complete medium;
- 2) adding a complete medium into a culture dish, putting a bowl-shaped silicone hydrogel carrier in the culture dish upside down, and incubating the bowl-shaped silicone hydrogel carrier in an incubator at 30-40° ° C. for 0.5-2 h;
- 3) taking another new culture dish, adding the complete medium into the new culture dish, taking out the silicone hydrogel carrier incubated in step 2), and placing the silicone hydrogel carrier vertically into the complete medium, with a bowl mouth facing up;
- 4) dissociating the umbilical cord mesenchymal stem cells cultured in step 1), performing resuspension uniformly, dripping an absorbed cell suspension into a concave surface of the bowl-shaped silicone hydrogel carrier, and placing the cells into an incubator for culture after the cells are settled;
- 5) performing periodic washing and exchange of the complete medium, performing washing after the umbilical cord mesenchymal stem cells overspread the silicone hydrogel carrier, transferring the silicone hydrogel carrier to a lenses access box filled with the complete medium, and sealing the silicone hydrogel carrier with a sealing film;
- and
- 6) irradiating the sealed bowl-shaped silicone hydrogel carrier to obtain lenses;
- wherein the bowl-shaped silicone hydrogel carrier is polymerized from an organosilicone monomer and a hydrophilic monomer under an action of a cross-linking agent and an initiator;
- the silicone monomer is polymerized from hydrogenated silicone oil and allyl methacrylate by a polymerization inhibitor and a catalyst.
Dependencies are inferred from the claim text.