US 12,616,218 B2 · Breast milk structured lipid simulating the composition of triglycerides and method for preparing the same

Abstract

The present invention provides a breast milk structured lipid simulating the composition of triglycerides and the method for preparing the same. The breast milk structured lipid exhibits a similarity of over 90% to breast milk lipid, including the contents of less than 0.3 mg/kg of esterified propenyl glycerol, less than 0.5 mg/kg of chloropropanol esters, and less than 0.3 g/100 g of trans fatty acids. The final product of the present invention exhibits a composition highly similar to breast milk while also meeting the requirements of the national food safety standard GB30604-2015.

A method for preparing a structured lipid simulating a composition of triglycerides in breast milk, wherein the method comprises the following steps:

based on fatty acid composition of breast milk lipid, determining a range of required sn-2 fatty acid composition during a lipid blending process and a range of required sn-1,3 fatty acid composition for an acidolysis reaction between a palm stearin and blended fatty acids, and establishing a lipid blending model to determine a molar ratio of selected lipids to the palm stearin, establishing an acidolysis equilibrium model to determine a composition of free fatty acids required for the acidolysis reaction under different substrate ratios, and establishing a fatty acid blending model to determine the proportions of fatty acids from different lipid sources;
performing the acidolysis reaction between the palm stearin and the blended fatty acids under the catalysis of a sn-1,3 lipase, wherein composition of the blended fatty acids is determined by the acidolysis equilibrium model and the fatty acid blending model, and a molar ratio of the palm stearin to the blended fatty acids is determined by the lipid blending model;
after the completion of the acidolysis reaction, performing fractionation and extraction in the presence of free fatty acids to adjust the content of tripalmitin in the acidolysis reaction product and remove a part of the glyceride, and removing the fatty acids via vacuum distillation to obtain a substitute lipid product similar to the breast milk lipid in terms of fatty acid composition and distribution, as well as the triglyceride composition,
wherein the lipid blending model is as follows:
sn
-
2
⁢
FA
⁢
%
=
Y
1
⁢
(
sn
-
2
)
+
∑
i
=
2
n
Y
i
⁡
(
sn
-
2
)
⁢
X
i
1
+
∑
i
=
2
n
X
i
,
(
1
)
wherein sn-2 FA % is the sn-2 fatty acid composition in mixed lipids, Y represents different fatty acids, Y1(sn-2) and Yi(sn-2) are molar percentages of each fatty acid at sn-2 position of the palm stearin and the lipids, respectively, Xi represents a molar ratio of the lipids to the palm stearin;
the compositions of total fatty acids and sn-1,3 fatty acids are also obtained, as shown below:
FA
⁢
%
=
Y
1
+
∑
i
=
2
n
Y
i
⁢
X
i
1
+
∑
i
=
2
n
X
i
,
(
2
)
sn
-
1.3
FA
⁢
%
=
3
×
(
Y
1
+
∑
i
=
2
n
Y
i
⁢
X
i
)
-
(
Y
1
⁢
(
sn
-
2
)
+
∑
i
=
2
n
Y
i
⁡
(
sn
-
2
)
⁢
X
i
)
2
×
(
1
+
∑
i
=
2
n
X
i
)
,
(
3
)
wherein FA % is the fatty acid composition in the mixed lipids, sn-1,3 FA % is the sn-1,3 fatty acid composition in the mixed lipids, Y1 and Yi are molar percentages of each fatty acid in the palm stearin and the lipids, respectively,
wherein the acidolysis equilibrium model is as follows:
the content of Xi fatty acids in the triglycerides sn-1,3 (sn-1/3Xi) at reaction equilibrium is as follows:
sn
-
1
/
3
⁢
FA
⁢
%
=
2
⁢
mM
sn
-
1
/
3
⁢
Xi
+
hM
Xi
∑
i
=
1
n
(
2
⁢
mM
sn
-
1
/
3
⁢
Xi
+
hM
Xi
)
,
(
4
)
wherein m is the amount of the triglycerides, h is the amount of the fatty acids, n is the type of the fatty acids, Xi represents different fatty acids, Msn-1/−3Xi is a molar percentage of Xi fatty acids on a sn-1,3 position of triglycerides, MXi is a molar percentage of free fatty acids Xi;
since
∑
i
=
1
n
M
sn
-
2
⁢
Xi
=
1
,
∑
i
=
1
n
M
Xi
=
1
,
and
⁢
therefore
∑
i
=
1
n
(
2
⁢
m
⁢
M
s
⁢
n
-
1
/
3
⁢
X
⁢
i
+
h
⁢
M
X
⁢
i
)
=
2
⁢
m
+
h
,
and the acidolysis equilibrium model is further simplified as:
sn
-
1
/
3
⁢
FA
⁢
%
=
2
⁢
mM
sn
-
1
/
3
⁢
Xi
+
hM
Xi
2
⁢
m
+
h
,
(
5
)
if h/m=S, a molar ratio between the free fatty acids to the triglycerides is S:1, and the content of Xi fatty acids in the triglycerides sn-1,3 at reaction equilibrium is further simplified as:
sn
-
1
/
3
⁢
FA
⁢
%
=
2
⁢
M
sn
-
1
/
3
⁢
Xi
+
SM
Xi
2
+
S
,
(
6
)
wherein the fatty acid blending model is as follows:
FA
⁢
%
=
∑
i
=
2
n
Y
i
⁢
X
i
∑
i
=
2
n
X
i
,
(
7
)
wherein Yi is a molar percentage of each fatty acid in the selected lipids, and Xi is a molar ratio of the selected lipids.

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Breast milk structured lipid simulating the composition of triglycerides and method for preparing the same

Life of this patent

Breast milk structured lipid simulating the composition of triglycerides and method for preparing the same

Life of this patent

Abstract

Claims7