US 12,614,320 B2Granted

Visualization tools for digital PCR data

AssigneeLife Technologies Corporation

InventorsHarrison Leong, Nivedita Sumi Majumdar, Jeffrey A. Marks, Theodore E. Straub, Ryan J. Talbot, Kevin S. Bodner, David Hoard

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Abstract

A method comprises displaying, on a graphical user interface (GUI), a first data visualization comprising a positional representation of a plurality of reaction sites from at least a portion of a substrate, wherein the positional representation of the first data visualization simultaneously indicates relative data quality of the plurality of reaction sites; and altering the first data visualization displayed on the GUI to a second data visualization, the second data visualization comprising the positional representation of the plurality of reaction sites from the at least the portion of the substrate, wherein the positional representation of the second data visualization simultaneously indicates relative data quality of the plurality of reaction sites from at least the portion of the substrate based on a comparison of the data quality values of the plurality of reaction sites an adjusted quality value threshold.

Claims19

Dependent claims

A method for generating a data visualization of data obtained from a biological sample analysis, the method comprising:

displaying, at a display comprising a graphical user interface, a scatter plot of data points corresponding to detected data from a plurality of sites of a substrate containing sample subjected to a biological analysis assay, the detected data comprising fluorescence emission intensity emitted from a plurality of dyes, wherein:
axes of the scatter plot correspond respectively to a first fluorescence emission intensity corresponding to a first dye of the plurality of dyes as a label for first amplified product of a first template nucleic acid, and to a second fluorescence emission intensity corresponding to a second dye of the plurality of dyes as a label for a second amplified product of a second template nucleic acid;
indicating, with a first indicator, a first cluster of data points of the scatter plot, the first cluster of data points corresponding to a first set of the plurality of sites classified as having no amplified product detected based on the detected fluorescence emission intensity;
indicating, with a second indicator, a second cluster of data points of the scatter plot, the second cluster of data points corresponding to a second set of the plurality of sites classified as having the first amplified product detected based on the detected fluorescence emission intensity;
indicating, with a third indicator, a third cluster of data points of the scatter plot, the third cluster of data points corresponding to a third set of the plurality of sites classified as having the second amplified product detected based on the detected fluorescence emission intensity;
and
indicating, with a fourth indicator, a fourth cluster of data points of the scatter plot, the fourth cluster of data points corresponding to a fourth set of the plurality of sites classified as having both the first amplified product and the second amplified product detected based on the detected fluorescence emission intensity,
wherein the first, second, third, and fourth indicators differ from each other.

5 more elements

A system for analysis of biological assay data, the system comprising:

a processor;
and
a memory encoded with instructions to:
display on a display comprising a graphical user interface, a scatter plot of data points corresponding to detected data from a plurality of sites of a substrate containing sample subjected to a biological analysis assay, the detected data comprising fluorescence emission intensity emitted from a plurality of dyes, wherein:
axes of the scatter plot correspond respectively to a first fluorescence emission intensity corresponding to a first dye of the plurality of dyes as a label for first amplified product of a first template nucleic acid, and to a second fluorescence emission intensity corresponding to a second dye of the plurality of dyes as a label for a second amplified product of a second template nucleic acid,
indicate, with a first indicator, a first cluster of data points of the scatter plot, the first cluster of data points corresponding to a first set of the plurality of sites classified as having no amplified product detected based on the detected fluorescence emission intensity;
indicate, with a second indicator, a second cluster of data points of the scatter plot, the second cluster of data points corresponding to a second set of the plurality of sites classified as having the first amplified product detected based on the detected fluorescence emission intensity;
indicate, with a third indicator, a third cluster of data points of the scatter plot, the third cluster of data points corresponding to a third set of the plurality of sites classified as having the second amplified product detected based on the detected fluorescence emission intensity;
and
indicate, with a fourth indicator, a fourth cluster of data points of the scatter plot, the fourth cluster of data points corresponding to a fourth set of the plurality of sites classified as having both the first amplified product and the second amplified product detected based on the detected fluorescence emission intensity,
wherein the first, second, third, and fourth indicators differ from each other.

8 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/861,854Filed 11 Jul 2022 · continuation
Priority: 7 Nov 2012
Published: US 2023/0022224 A126 Jan 2023
Granted: 28 Apr 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification6 codes

G06T 11/26
Drawing from basic elements: drawing of charts or graphs
G16B 25/20
ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression: polymerase chain reaction [PCR]; Primer or probe…
G06T 11/23
Drawing from basic elements: using straight lines or curves
G16B 45/00
Ict specially adapted for bioinformatics-related data visualisation

Family

Application61/723,732Us provisional application · filed 7 Nov 2012
Application61/803,010Us provisional application · filed 18 Mar 2013
PCT/US2013/068984Parent of this continuation · filed 7 Nov 2013
Application16/937,634Parent of this continuation · filed 24 Jul 2020
US 11,417,037 B2Parent of this continuation · filed 28 Jul 2020
US 2023/0022224 A1Pre-grant publication · published 26 Jan 2023
Application14/441,500Parent of this continuation
US 10,726,589 B2Parent of this continuation
US 2015/0269756 A1Published application
US 2021/0042972 A1Published application
US 12,614,320 B2This patent

From the US filing history. Foreign family members aren’t listed.