Computer-implemented method for quality control of a digital image of a sample

A system comprising:

• one or more data processors;
• and
• a non-transitory computer readable storage medium containing instructions which, when executed on the one or more data processors, cause the one or more data processors to perform a set of actions including:
• accessing a z-stack comprising a plurality of digital images of a sample, wherein each digital image of the plurality of digital images is a two-dimensional image depicts a sample mounted on a slide and corresponds to a different focal distance between the slide and an imaging device of a slide imaging apparatus;
• automatically determining, for each digital image in the z-stack, sharpness values of sub-regions of at least one region of interest of the digital image by using at least one edge detection image filters;
• comparing the sharpness values for each sub-regions of the at least one region of interest across the plurality of digital images;
• selecting in-focus regions of each digital images in the z-stack based on the comparison;
• and
• automatically generating a three-dimensional digital image based on the selected in-focus regions.

A computer-program product tangibly embodied in a non-transitory machine-readable storage medium, including instructions configured to cause one or more data processors to perform a set of actions including:

• accessing a z-stack comprising a plurality of digital images of a sample, wherein each digital image of the plurality of digital images is a two-dimensional image depicts a sample mounted on a slide and corresponds to a different focal distance between the slide and an imaging device of a slide imaging apparatus;
• automatically determining, for each digital image in the z-stack, sharpness values of sub-regions of at least one region of interest of the digital image by using at least one edge detection image filter;
• comparing the sharpness values for each sub-regions of the at least one region of interest across the plurality of digital images;
• selecting in-focus regions of each digital images in the z-stack based on the comparison;
• and
• automatically generating a three-dimensional digital image based on the selected in-focus regions.