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US 12,613,245 B2Granted
Methods for detecting and inhibiting brain metastasis
AssigneeCornell University
InventorsDavid C. Lyden, Goncalo Rodrigues
Life of this patent
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Abstract
The present disclosure is directed to a method that involves selecting a subject having a primary tumor and obtaining, from the selected subject, a sample containing exosomes derived from primary tumor cells. The exosomes are isolated from the sample and expression levels of cell migration-inducing and hyaluronan-binding protein (CEMIP) are detected in the isolated exosomes.
Claims19
A method comprising:
- selecting a subject having a primary tumor;
- obtaining, from the selected subject, a sample containing tumor-derived exosomes from primary tumor cells, wherein the tumor-derived exosomes carry tumor-associated surface molecules or lack CD45, CD80, and CD86 expression;
- isolating and/or enriching the tumor-derived exosomes from said sample based on said surface molecules or lack thereof;
- and
- detecting, in said isolated tumor-derived exosomes, expression levels of cell migration-inducing and hyaluronan-binding protein (CEMIP).
2 more elements
A method of identifying a subject's risk of developing metastatic brain disease, said method comprising:
- selecting a subject having a primary tumor;
- isolating and/or enriching, from said subject, a sample comprising tumor-derived exosomes derived from primary tumor cells, wherein the tumor-derived exosomes carry tumor-associated surface molecules or lack CD45, CD80, and CD86 expression;
- detecting an increase in cell migration-inducing and hyaluronan-binding protein (CEMIP) expression in said tumor-derived exosomes relative to average CEMIP expression in a subject with metastasis to organ sites other than the brain, or without metastasis;
- prognosing that the subject has an increased risk of developing metastatic brain disease based on said detecting increased expression of CEMIP;
- and
- administering, to the subject prognosed to have an increased risk of developing metastatic brain disease, a brain metastasis prophylactic treatment suitable for inhibiting metastatic brain disease.
3 more elements
A method of inhibiting metastatic brain disease in a subject, said method comprising:
- selecting a subject having a primary tumor,
- isolating and/or enriching, from said subject, a sample comprising tumor-derived exosomes derived from primary tumor cells, wherein the tumor-derived exosomes carry tumor-associated surface molecules or lack CD45, CD80, and CD86 expression, and wherein expression level of cell migration-inducing and hyaluronan-binding protein (CEMIP) from said exosomes derived from primary tumor cells is increased relative to average CEMIP expression levels in exosomes derived from non-tumor cells, and
- administering, to the selected subject, a brain metastasis prophylactic treatment suitable for inhibiting metastatic brain disease.
Dependencies are inferred from the claim text.