Immunochromatographic test strip

An immunochromatographic detection method which comprises:

• providing an immunochromatographic test strip comprising a sample pad serving as a sample application region, a conjugate pad or a conjugate part in a portion of the sample pad, a porous membrane, a top film, and an opaque base material, the porous membrane including at least a detection region with the opaque base material placed only on a back surface of the porous membrane, wherein the top film is covering an uppermost surface of the immunochromatographic test strip so as to cover the detection region immobilized on the front surface of the porous membrane, but a portion of the porous membrane is exposed, and
• wherein a backing sheet is placed under the sample pad, the conjugate pad or the conjugate part, and the opaque base material, and the thickness of the opaque base material is 80 μm or more and 120 μm or less;
• applying a sample to the immunochromatographic test strip;
• and
• detecting an optical parameter derived from a labeling substance by an apparatus.

A method for reducing variation in measurement values in an immunochromatographic detection method which comprises:

• providing an immunochromatographic test strip comprising at least a sample pad serving as a sample application region, a conjugate pad or a conjugate part in a portion of the sample pad, a porous membrane, a top film, and an opaque base material, wherein the immunochromatographic test strip includes a development region and a detection region, wherein the detection region is immobilized on a front surface of the porous membrane, wherein the top film is covering an uppermost surface of the immunochromatographic test strip so as to cover the detection region immobilized on the front surface of the porous membrane, but a portion of the porous membrane is exposed, and wherein the opaque base material is placed only on a back surface of the porous membrane,
• wherein a backing sheet is placed under the sample pad, the conjugate pad or the conjugate part, and the opaque base material, and the thickness of the opaque base material is 80 μm or more and 120 μm or less;
• applying a sample to the immunochromatographic test strip;
• and
• detecting an optical parameter derived from a labeling substance by an apparatus.

A method for reducing bleaching of a test strip in an immunochromatographic detection method which comprises:

• providing immunochromatographic test strip comprising at least a sample pad serving as a sample application region, a conjugate pad or a conjugate part in a portion of the sample pad, a porous membrane, a top film, and an opaque base material, wherein the immunochromatographic test strip includes a development region, and a detection region, wherein the detection region is immobilized on a front surface of the porous membrane, wherein the top film is covering an uppermost surface of the immunochromatographic test strip so as to cover the detection region immobilized on the front surface of the porous membrane, but a portion of the porous membrane is exposed, and wherein the opaque base material is placed only on a back surface of the porous membrane,
• wherein a backing sheet is placed under the sample pad, the conjugate pad or the conjugate part, and the opaque base material, and the thickness of the opaque base material is 80 μm or more and 120 μm or less;
• applying a sample to the immunochromatographic test strip;
• and
• detecting an optical parameter derived from a labeling substance by an apparatus.