Immunochromatography

Immunochromatography method comprising:

• mixing an antigen-containable specimen and modified magnetic particles, which are magnetic particles modified with a monoclonal antibody X having a specific affinity to the antigen, to obtain a mixture containing magnetic particle composite bodies which are composite bodies of the antigen and the modified magnetic particle;
• collecting the magnetic particle composite bodies in the mixture using magnetism;
• dissociating the modified magnetic particles from the magnetic particle composite body to obtain an antigen-containing solution by mixing the collected magnetic particle composite bodies with a dissociation solution which is an acidic or alkaline solution;
• neutralizing the antigen-containing solution using a neutralization solution to obtain a neutralized antigen-containing solution;
• spreading gold particle composite bodies on an insoluble carrier, in a state where the gold particle composite bodies which are composite bodies of the antigen and modified gold particles are formed, by adding dropwise the neutralized antigen-containing solution onto a gold colloid holding pad, which is a pad that holds the modified gold particles which are gold particles modified with a monoclonal antibody Y capable of binding to an antigen in the neutralized antigen-containing solution, of the insoluble carrier having the gold colloid holding pad and a reaction site at which a monoclonal antibody Z capable of binding to the antigen has been immobilized;
• capturing the gold particle composite bodies at the reaction site of the insoluble carrier;
• and
• silver-amplifying the captured gold particle composite body,
• wherein the monoclonal antibody X and the monoclonal antibody Y are different from each other, and
• the monoclonal antibody X and the monoclonal antibody Z are different from each other.