Biosensors for detecting arrestin signaling
AssigneeMontana Molecular LLC
InventorsThomas Hughes, Paul Tewson
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Abstract
Disclosed herein are nucleic acids comprising fluorescent reporter constructs for detecting changes in the arrestin cellular signaling changes in a cell. Also provided are methods for detecting cellular arrestin signaling changes in cells, as well as vectors and cells comprising nucleic acids comprising reporter constructs for detecting cellular arrestin signaling changes in a cell.
Claims10
A nucleic acid encoding a fusion protein, wherein the fusion protein consists of an arrestin protein, a linker, and a single circularly permuted fluorescent protein;
- wherein the fusion protein undergoes a change in fluorescence upon association with an intracellular portion of a G-protein-coupled receptor,
- wherein the circularly permuted fluorescent protein is at least 95% identical to SEQ ID NO:
- 5, and
- wherein the linker consists of an amino acid sequence selected from the group consisting of Gly, Pro-Ser-His, Met-Arg-Gly, SEQ ID NO:
- 46, SEQ ID NO:
- 57, and Asn-Val.
A fusion protein consisting of an arrestin protein, a linker, and a single circularly permuted fluorescent protein, wherein the fusion protein undergoes a change in fluorescence upon association with an intracellular portion of a G-protein-coupled receptor, wherein the circularly permuted fluorescent protein is at least 95% identical to SEQ ID NO:
- 5, and wherein the linker consists of an amino acid sequence selected from the group consisting of Gly, Pro-Ser-His, Met-Arg-Gly, SEQ ID NO:
- 46, SEQ ID NO:
- 57, and Asn-Val.
Dependencies are inferred from the claim text.