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US 12,613,236 B2Granted
Lung fibrosis model and methods of using the same
AssigneeDuke University
InventorsPurushothama Rao Tata, Aleksandra Tata, Arvind Konkimalla, Yoshihiko Kobayashi
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Abstract
The present disclosure provides a newly-identified transitional cell state in alveolar regeneration, models to ablate lung alveolar type-1 cells that leads to lung fibrosis and emphysema, a scalable, an ex vivo lung fibrosis model that uses co-cultured lung fibroblasts and pre-alveolar type-1 transitional cell state (PATS) for the use of disease modeling and drug screening, and methods of using same.
Claims15
A method for drug screening on a lung injury organoid model, the method comprising:
- providing in a culture medium a co-culture of cells comprising pre-alveolar type-1 transitional cell state (PATS) cells and alveolar fibroblasts;
- and
- screening a drug for a biological effect by
- contacting the culture medium with a drug;
- and
- measuring accumulation of PATS cells and/or expression of at least one marker of PATS cells.
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