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US 12,613,234 B2Granted
Structural-profiling of analytes by nanopore trapping
AssigneeNanjing University
InventorsShuo Huang, Yao Liu, Yuqin Wang, ShanYu Zhang
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AssigneeNanjing University
InventorsShuo Huang, Yao Liu, Yuqin Wang, ShanYu Zhang
Estimating the term…
A method of characterizing an analyte or the interaction between the analyte and an agent in a nanopore system, wherein the nanopore system comprises a protein nanopore disposed in a membrane that separates a first conductive liquid medium from a second conductive liquid medium, wherein the protein nanopore is MspA, MspA homolog or variant thereof, wherein the analyte has an conformation and the analyte with the conformation can be accommodated in the vestibule of the MspA, the MspA homolog or the variant thereof but cannot translocate through the MspA, the MspA homolog or the variant thereof, the method comprising: i) applying an electrical potential difference between the first conductive liquid medium and the second conductive liquid medium to drive the analyte into the nanopore, and optionally contacting the agent with the analyte; ii) measuring an ionic current through the protein nanopore to provide a tested current pattern that contains at least ionic current measured during the analyte is in the vestibule of the MspA, the MspA homolog or the variant thereof; iii) associating the tested current pattern with at least one characteristic of the analyte or the interaction between the analyte and an agent.
A method of characterizing an analyte in a nanopore system, wherein the nanopore system comprises a protein nanopore disposed in a membrane that separates a first conductive liquid medium from a second conductive liquid medium, wherein the protein nanopore is MspA, MspA homolog or variant thereof, wherein the analyte has a conformation and the analyte with the conformation can be accommodated in the vestibule of the MspA, the MspA homolog or the variant thereof but cannot translocate through the MspA, the MspA homolog or the variant thereof, the method comprising:
A method for characterizing the interaction between an analyte and an agent or the agent capable of interacting with the analyte in a nanopore system, wherein the nanopore system comprises a protein nanopore disposed in a membrane that separates a first conductive liquid medium from a second conductive liquid medium, wherein the protein nanopore is MspA, MspA homolog or variant thereof, wherein the analyte has a conformation and the analyte with the conformation can be accommodated in the vestibule of the MspA, the MspA homolog or the variant thereof but cannot translocate through the MspA, the MspA homolog or the variant thereof, the method comprising:
A method for detecting an analyte of interest in a sample in a nanopore system, wherein the nanopore system comprises a protein nanopore disposed in a membrane that separates a first conductive liquid medium from a second conductive liquid medium, wherein the protein nanopore is MspA, the MspA homolog or the variant thereof, wherein the analyte of interest has a conformation and the analyte of interest with the original conformation can be accommodated in the vestibule of MspA, the MspA homolog or the variant thereof but cannot translocate through MspA, the MspA homolog or the variant thereof, the method comprising:
Dependencies are inferred from the claim text.