Method in bioprocess purification system

A control system for reconstructing events during non-method time related to a previously performed process run in a bioprocess purification system, the events during non-method time being controlled by a number of instructions executed consecutively in a program, wherein the control system is configured to interact with hardware configured to control the events during non-method time related to the purification of a liquid containing a sample in the bioprocess purification system, wherein the control system is further configured to, during the process run:

• record hardware state related to the process run as a function of time and/or volume of the process run;
• modify the hardware state of the bioprocess purification system during the non-method time, wherein the modifying comprises changing a configuration of one or more valves and/or one or more pumps of the bioprocess purification system between a first portion of method time during the process run and a second portion of method time during the process run;
• record readings from UV and/or conductivity sensors related to the process run as a function of time and/or volume of the process run, wherein the UV and/or conductivity sensors are configured to record sensor data during the method time and not during the non-method time;
• record manual instructions executed during the process run as a function of time and/or volume of the process run;
• synchronize the hardware state with the readings from sensors to link the hardware state with the result from the process run;
• and
• synchronize the hardware state and the readings from sensors with the manual instructions to link the instructions with the hardware state and,
• wherein the control system is further configured to, after completion of the process run, (i) determine, based at least in part on the hardware state, a non-allowed hardware combination of the bioprocess purification system during the first section of the non-method time, (ii) wherein the hardware state and the readings from the sensors were synchronized with the manual instructions to link a particular instruction of the instructions to the non-allowed hardware combination, and (iii) display the hardware state, readings, and manual instructions executed during the process run as a function of time and/or volume of the previously performed process run on a graphical user interface configured to show:
• a control bar, comprising:
• (i) a first section that corresponds to the first portion of method time, (ii) a second section that corresponds to the non-method time, and (iii) a third section that corresponds to the second portion of method time;
• a cursor on the control bar, wherein the cursor can be placed at any point on the control bar by a user;
• a chromatogram of the readings recorded from the UV and/or conductivity sensors related to the process run;
• a first graphical element on the chromatogram corresponding to a location of the cursor and representing a UV or conductivity reading, wherein the first graphical element remains unchanged when the cursor is moved by the user between any two points within the non-method time;
• a second graphical element corresponding to the hardware state, wherein the second graphical element depicts a first hardware state when the cursor is moved to a first point in the non-method time and a second hardware state different from the first hardware state when the cursor is moved to a second point in the non-method time;
• and
• a third graphical element for identifying that the bioprocess purification system was in the non-allowed hardware combination during the first section of the non-method time and that non- allowed hardware combination emanated from the particular instruction.