Method for detecting and quantifying analytes in a microfluidic device

A microfluidic device (1) comprising:

• at least one fluid channel (2) comprising at least one inlet (3), wherein said at least one fluid channel (2) is fluidly connected to a first sensor (4) downstream of said at least one fluid inlet (3), wherein the first sensor (4) comprises at least one sensor cathode (5) and at least one sensor anode (6) formed on an electrically isolating substrate (7), wherein the sensor cathode (5) and the sensor anode (6) are spaced apart by a gap (8) formed on the electrically isolating substrate (7), and wherein at least one analyte capturing molecule (9) is immobilized in the gap (8) on the substrate (7), wherein the at least one capturing molecule (9) is adapted to capture at least one analyte (10) of a fluid sample introduced into the inlet (3) and transported to the first sensor (4), wherein the microfluidic device (1) comprises at least one second sensor (11) fluidly connected to the fluid channel (2) or to said first sensor (4), wherein the at least one second sensor (11) comprises at least one sensor cathode (5) and at least one sensor anode (6) formed on the electrically isolating substrate (7), wherein the sensor cathode (5) and the sensor anode (6) are spaced apart by a gap (8) formed on the electrically isolating substrate (7), and wherein at least one capture molecule (9) is immobilized in the gap (8) on the substrate (7), which is adapted to capture at least one analyte (10) of a sample introduced into the inlet (3) and transported to the second sensor (11), wherein the gap (8) between the sensor cathode (5) and the sensor anode (6) of the at least one second sensor (11) varies in size compared to the gap (8) of the first sensor (4), wherein the at least one second sensor (11) is connected to the fluid channel (2) downstream of the first sensor (4).